diff --git a/changelog.d/10415.misc b/changelog.d/10415.misc
new file mode 100644
index 000000000..3b9501acb
--- /dev/null
+++ b/changelog.d/10415.misc
@@ -0,0 +1 @@
+Remove shebang line from module files.
diff --git a/synapse/_scripts/review_recent_signups.py b/synapse/_scripts/review_recent_signups.py
index 01dc0c423..9de913db8 100644
--- a/synapse/_scripts/review_recent_signups.py
+++ b/synapse/_scripts/review_recent_signups.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2021 The Matrix.org Foundation C.I.C.
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/admin_cmd.py b/synapse/app/admin_cmd.py
index 2878d2c14..3234d9ebb 100644
--- a/synapse/app/admin_cmd.py
+++ b/synapse/app/admin_cmd.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2019 Matrix.org Foundation C.I.C.
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/appservice.py b/synapse/app/appservice.py
index 2d50060ff..de1bcee0a 100644
--- a/synapse/app/appservice.py
+++ b/synapse/app/appservice.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/client_reader.py b/synapse/app/client_reader.py
index 2d50060ff..de1bcee0a 100644
--- a/synapse/app/client_reader.py
+++ b/synapse/app/client_reader.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/event_creator.py b/synapse/app/event_creator.py
index 57af28f10..885454ed4 100644
--- a/synapse/app/event_creator.py
+++ b/synapse/app/event_creator.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2018 New Vector Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/federation_reader.py b/synapse/app/federation_reader.py
index 2d50060ff..de1bcee0a 100644
--- a/synapse/app/federation_reader.py
+++ b/synapse/app/federation_reader.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/federation_sender.py b/synapse/app/federation_sender.py
index 2d50060ff..de1bcee0a 100644
--- a/synapse/app/federation_sender.py
+++ b/synapse/app/federation_sender.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/frontend_proxy.py b/synapse/app/frontend_proxy.py
index 2d50060ff..de1bcee0a 100644
--- a/synapse/app/frontend_proxy.py
+++ b/synapse/app/frontend_proxy.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/generic_worker.py b/synapse/app/generic_worker.py
index c3d499251..3b7131af8 100644
--- a/synapse/app/generic_worker.py
+++ b/synapse/app/generic_worker.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 # Copyright 2020 The Matrix.org Foundation C.I.C.
 #
diff --git a/synapse/app/homeserver.py b/synapse/app/homeserver.py
index 920b34d97..7dae163c1 100644
--- a/synapse/app/homeserver.py
+++ b/synapse/app/homeserver.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2014-2016 OpenMarket Ltd
 # Copyright 2019 New Vector Ltd
 #
diff --git a/synapse/app/media_repository.py b/synapse/app/media_repository.py
index 2d50060ff..de1bcee0a 100644
--- a/synapse/app/media_repository.py
+++ b/synapse/app/media_repository.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/pusher.py b/synapse/app/pusher.py
index 2d50060ff..de1bcee0a 100644
--- a/synapse/app/pusher.py
+++ b/synapse/app/pusher.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/synchrotron.py b/synapse/app/synchrotron.py
index 2d50060ff..de1bcee0a 100644
--- a/synapse/app/synchrotron.py
+++ b/synapse/app/synchrotron.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/app/user_dir.py b/synapse/app/user_dir.py
index a368efb35..14bde2717 100644
--- a/synapse/app/user_dir.py
+++ b/synapse/app/user_dir.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2017 Vector Creations Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/push/pusherpool.py b/synapse/push/pusherpool.py
index 85621f33e..a1436f393 100644
--- a/synapse/push/pusherpool.py
+++ b/synapse/push/pusherpool.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2015, 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/synapse/util/versionstring.py b/synapse/util/versionstring.py
index dfa30a622..cb08af738 100644
--- a/synapse/util/versionstring.py
+++ b/synapse/util/versionstring.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # Copyright 2016 OpenMarket Ltd
 #
 # Licensed under the Apache License, Version 2.0 (the "License");